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This textbook introduces the fundamental methods, tools and approaches in protein science, covering various aspects of proteins across multiple disciplines to best understand what proteins are. In an age of AI and instant access to vast electronic databases, the challenge is no longer finding information, but understanding how the pieces fit together. This textbook builds on the success of its original Hungarian edition 1000 arcu feherjek, published in 2018 by Semmelweis Kiado. This updated English edition contains additional didactic elements, including exercises, to enhance the learning experience. Chapters by expert contributors include key topics such as stability of protein samples, protein engineering, structural testing through diffraction techniques, free electron laser applications, protein crystallography, NMR, ESR spectroscopy, and cryo-electron microscopy. Particular attention is given to electron and vibrational spectroscopy in protein research, fluorescent modification, and spectroscopic studies of proteins, as well as the examination of protein molecules through atomic force microscopy. Extending its scope to cellular protein research, the textbook explores light microscopy, signature-free interaction measurement, rapid kinetic methods, and techniques based on the coupling of antigens and antibodies. Readers will discover valuable insights into electrophoretic methods, examination of protein networks, and mass spectrometry-based proteomics. The concluding chapter addresses the challenges and approaches involved in the scale-up and industrial production of recombinant proteins.

This textbook serves as a step-by-step guide to protein science, encompassing topics from recombinant production, purification, and identification of proteins to advanced techniques such as chemical synthesis and modification. With its didactic language and cutting-edge scientific content, the book is an indispensable resource for students, researchers, and professionals alike. The English edition was initially prepared with the assistance of artificial intelligence to provide a consistent linguistic and stylistic foundation across the contributions of the many authors. The individual chapters were then extensively revised, updated, and expanded by the authors, resulting in a substantially enriched and up-to-date English edition that brings together the latest developments across the field.