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This book provides a comprehensive introduction to equilibrium and non-equilibrium Green's function methods in many-body physics. It begins with a derivation of second quantisation for relativistic systems based on the many-body relativistic Dirac equation and its non-relativistic limit. The properties of equilibrium Green's functions are then described, with discussion of the two-time and Matsubara Green's function methods. The coverage of non-equilibrium Green's function methods includes the diagrammatic techniques applicable to electrons and phonons using both the perturbation and variational approaches. Specific applications to steady-state and time-dependent quantum transport are presented in the final chapters. The book's accessible explanations, detailed derivations, and systematic treatment of the underlying theory make it a valuable resource for graduate students and early-career researchers. More than 200 problems have been included to support learning, with selected solutions available at the end of each chapter. Instructors benefit from access to the full solutions manual.