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This fully updated volume explores methods to create and use nanostructures for characterizing, targeting, and controlling the behavior of cells. Experts from around the globe present their procedures for designing, preparing, characterizing, and using DNA nanostructures, especially towards biological or materials science applications. Additionally, the study of fundamental aspects of nucleic acids, such as their pairing and stacking energy measured with optical tweezers, as well as the targeted delivery of lipid-encapsulated nucleic acid drugs, is also provided to complete the landscape of nanoscale nucleic acids methods. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, DNA Nanotechnology: Methods and Protocols, Third Edition serves as an ideal resource for researchers working on applications of DNA nanostructures in biotechnology and nanomedicine.