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CRISPR-Cas Methods. 2020 ed.
・ISBN 978-1-0716-0618-6 paper EUR 119.99
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| 著者・編者 | Islam, M. Tofazzal / Bhowmik, Pankaj K. / Molla, Kutubuddin A. (eds.), |
|---|---|
| シリーズ | (Springer Protocols Handbooks) |
| 出版社 | (Springer-Verlag New York Inc., US) |
| 出版年月 | 2020 |
| ページ数 | 278 pp. |
| 言語 | ENG |
| ニュース番号 | ＜A02-78366＞ |
解説
This volume details the fundamentals of the CRISPR-Cas system, and its protocols illustrate advances in CRISPR-Cas techniques for efficient genome editing. Introductory chapters provide a wide horizon of CRISPR/Cas-based methods and applications. Additional chapters guide readers through HDR-mediated editing, sgRNA design, the step-by-step procedure of multiplex adenine base editing experiments in rice, generating mutants for rice, wheat, Brachypodium, Barley, Flax, and Phytophthora, visual screening of mutants, gene deletion (knock-out), tagging (knock-in) in mammalian cells, the cloning-free (DNA-free) technique, cell-penetrating peptides, generating a genome-edited banana, and nuclear genome editing of Chlamydomonas employing CRISPR-Cpf1 combined with a single-stranded DNA (ssODN) repair template.
Authoritative and cutting-edge, CRISPR-Cas Methods aims to assist researchers who are new to the field and are aiming to learn how best to adopt this technology for a particular organism.
Authoritative and cutting-edge, CRISPR-Cas Methods aims to assist researchers who are new to the field and are aiming to learn how best to adopt this technology for a particular organism.