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This dissertation covers several important aspects of relativistically intense laser-microplasma interactions and some potential applications. A Paul-trap based target system was developed to provide fully isolated, well defined and well positioned micro-sphere-targets for experiments with focused peta-watt laser pulses. The laser interaction turned such targets into microplasmas, emitting proton beams with kinetic energies exceeding 10 MeV. The proton beam kinetic energy spectrum and spatial distribution were tuned by variation of the acceleration mechanism, reaching from broadly distributed spectra in relatively cold plasma expansions to spectra with relative energy spread as small as 20% in spherical multi-species Coulomb explosions and in directed acceleration processes. Numerical simulations and analytical calculations support these experimental findings and show how microplasmas may be used to engineer laser-driven proton sources. In a secondeffort, tungsten micro-needle-targets were used at a peta-watt laser to produce few-keV x-rays and 10-MeV-level proton beams simultaneously, both measured to have only few-?m effective source-size. This source was used to demonstrate single-shot simultaneous radiographic imaging with x-rays and protons of biological and technological samples.
Finally, the dissertation discusses future perspectives and directions for laser-microplasma interactions including non-spherical target shapes, as well as thoughts on experimental techniques and advanced quantitative image evaluation for the laser driven radiography.