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DNA Modification Detection Methods. 2022 ed.
・ISBN 978-1-0716-1231-6 paper EUR 129.99
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| 著者・編者 | Yuan, Bi-Feng (ed.), |
|---|---|
| シリーズ | (Springer Protocols Handbooks) |
| 出版社 | (Springer-Verlag New York Inc., US) |
| 出版年月 | 2022 |
| ページ数 | 194 pp. |
| 言語 | ENG |
| ニュース番号 | ＜A02-74750＞ |
解説
Understanding the functional roles of DNA modifications relies on the accurate detection, quantification, and mapping of DNA modifications. Methods for deciphering DNA modifications have substantially improved over the last several years, which greatly revolutionize the field of DNA modifications. In addition to DNA cytosine methylation (5-methylcytosine, 5mC), the best-characterized epigenetic modification, many new modifications have been discovered to present in DNA in recent years.
This book provides a comprehensive overview of available techniques and methods together with detailed step-by-step protocols for experimental procedures required to successfully perform analysis on various types of DNA modifications, including 5mC, 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC), 5-carboxycytosine (5caC), 5-hydroxymethyluracil (5hmU), 5-formyluracil (5fU), N6-methyladenine (6mA), ?-D-glucosyl-5-hydroxymethyluracil (base J) and 8-oxo-7,8-dihydroguanine (OG). This laboratory manual is a valuable source for biochemists and molecular biologists from different fields who wish to investigate DNA modifications.