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This volume provides readers with a comprehensive collection of methods to guide them on how to generate, characterize, and use naive human pluripotent stem cells (hPSCs). The chapters in this book cover topics such as three predominant routes to generate naive hPSC lines; methods to differentiate naive hPSCs into specialized cell types; and techniques to characterize naive hPSCs using key molecular landmarks that benchmark and quality control the cell lines. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Human Naive Pluripotent Stem Cells: Methods and Protocols is a valuable resource for novice and expert researchers who are looking to learn more or expand their research in this developing field.