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Genetically Incorporated Non-Canonical Amino Acids: Methods and Protocols.
・ISBN 978-1-0716-3250-5 hard EUR 179.99
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| 著者・編者 | Tsai, Yu-Hsuan / Elsaesser, Simon J. (eds.), |
|---|---|
| シリーズ | (Methods in Molecular Biology 2676) |
| 出版社 | (Springer-Verlag New York Inc., US) |
| 出版年月 | 2023 |
| ページ数 | 286 pp. |
| 言語 | ENG |
| ニュース番号 | <A02-17313> |
解説
This detailed volume explores non-canonical amino acids (ncAAs) through their site-specific incorporation by genetic code expansion (GCE). The collection provides a broad resource of methods for implementing GCE in E. coli, mammalian cells, and animals, highlighting specific applications ranging from fluorescence labeling to photocontrol and the study of protein post-translational modification. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Genetically Incorporated Non-Canonical Amino Acids: Methods and Protocols serves as an ideal source of methodologies that can be adapted and extended, migrated to different model systems, and combined in new ways to help explore a wide range of biological questions and to augment industrial and pharmaceutical protein engineering.
Authoritative and practical, Genetically Incorporated Non-Canonical Amino Acids: Methods and Protocols serves as an ideal source of methodologies that can be adapted and extended, migrated to different model systems, and combined in new ways to help explore a wide range of biological questions and to augment industrial and pharmaceutical protein engineering.