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The seven-volume set LNCS 12261, 12262, 12263, 12264, 12265, 12266, and 12267 constitutes the refereed proceedings of the 23rd International Conference on Medical Image Computing and Computer-Assisted Intervention, MICCAI 2020, held in Lima, Peru, in October 2020. The conference was held virtually due to the COVID-19 pandemic.

The 542 revised full papers presented were carefully reviewed and selected from 1809 submissions in a double-blind review process. The papers are organized in the following topical sections:

Part I: machine learning methodologies

Part II: image reconstruction; prediction and diagnosis; cross-domain methods and reconstruction; domain adaptation; machine learning applications; generative adversarial networks

Part III: CAI applications; image registration; instrumentation and surgical phase detection; navigation and visualization; ultrasound imaging; video image analysis

Part IV: segmentation; shape models and landmark detection

Part V: biological, optical, microscopic imaging; cell segmentation and stain normalization; histopathology image analysis; opthalmology

Part VI: angiography and vessel analysis; breast imaging; colonoscopy; dermatology; fetal imaging; heart and lung imaging; musculoskeletal imaging

Part VI: brain development and atlases; DWI and tractography; functional brain networks; neuroimaging; positron emission tomography