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PCR Protocols in Molecular Toxicology

PCR Protocols in Molecular Toxicology

・ISBN 978-0-8493-3344-6 paper GB£ 115.00

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電子版あり 大学・学術機関向け電子ブック（eBook）ISBN 9780429186936

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著者・編者Vanden Heuvel, John P.,
シリーズ (Methods in Life Sciences - Toxicology Section)
出版社 (CRC Press Inc, US)
出版年月1997
ページ数252 pp.
言語ENG
ニュース番号＜A00-56449＞

解説

Molecular toxicology is an emerging discipline that utilizes molecular and cell biology to understand how drugs and chemicals result in their unwanted effects. PCR Protocols in Molecular Toxicology is a practical guide to the use of polymerase chain reaction (PCR) to help examine, on a molecular and cellular level, how toxic responses are manifested. It offers a basic understanding of PCR and its optimization, as well as describing specific, high-impact areas of molecular toxicology and recent advances. The following techniques are described in detail:

  • Quantitative reverse transcriptase PCR and methods to examine gene expression
  • Differential display cloning
  • Cloning and library screening by PCR
  • Genotype and polymorphism analysis of drug and toxicant metabolizing enzymes
  • Basic, non-PCR based molecular biology methods

    PCR Protocols in Molecular Toxicology will aid both novices and experienced PCR practitioners in using PCR to its fullest potential.