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The science of networks represented a substantial change in the way we see natural and technological phenomena. Now we have a better understanding that networks are, in most cases, networks of networks or multi-layered networks. This book provides a summary of the research done during one of the largest and most multidisciplinary projects in network science and complex systems (Multiplex). The science of complex networks originated from the empirical evidence that most of the structures of systems such as the internet, sets of protein interactions, and collaboration between people, share (at least qualitatively) common structural properties. This book examines how properties of networks that interact with other networks can change dramatically. The authors show that, dependent on the properties of links that interconnect two or more networks, we may derive different conclusions about the function and the possible vulnerabilities of the overall system of networks. This book presents a series of novel theoretical results together with their applications, providing a comprehensive overview of the field.