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Many deep concerns in the life sciences and medicine have to do with the enactment, ordering and displacement of a broad range of values. This volume articulates a pragmatist stance for the study of the making of values in society, exploring various sites within life sciences and medicine and asking how values are at play. This means taking seriously the work scientists, regulators, analysts, professionals and publics regularly do, in order to define what counts as proper conduct in science and health care, what is economically valuable, and what is known and worth knowing. A number of analytical and methodological means to investigate these concerns are presented. The editors introduce a way to indicate an empirically oriented research program into the enacting, ordering and displacing of values. They argue that a research programme of this kind, makes it possible to move orthogonally to the question of what values are, and thus ask how they are constituted. This rectifies some central problems that arise with approaches that depend on stabilized understandings of value. At the heart of it, such a research programme encourages the examination of how and with what means certain things come to count as valuable and desirable, how registers of value are ordered as well as displaced. It further encourages a sense that these matters could be, and sometimes simultaneously are, otherwise.