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The arrival of English settlers in the American Southeast in 1670 brought the British and the Native Americans into contact both with foreign peoples and with unfamiliar gender systems. In a region in which the balance of power between multiple players remained uncertain for many decades, British and Native leaders turned to concepts of gender and family to create new diplomatic norms to govern interactions as they sought to construct and maintain working relationships. In Brothers Born of One Mother, Michelle LeMaster addresses the question of how differing cultural attitudes toward gender influenced Anglo-Indian relations in the colonial Southeast.

As one of the most fundamental aspects of culture, gender had significant implications for military and diplomatic relations. Understood differently by each side, notions of kinship and proper masculine and feminine behaviour wielded during negotiations had the power to either strengthen or disrupt alliances. The collision of different cultural expectations of masculine behaviour and men's relationships to and responsibilities for women and children became significant areas of discussion and contention. Native American and British leaders frequently discussed issues of manhood (especially in the context of warfare), the treatment of women and children, and intermarriage. Women themselves could either enhance or upset relations through their active participation in diplomacy, war, and trade.

Leaders invoked gendered metaphors and fictive kinship relations in their discussions, and by evaluating their rhetoric, Brothers Born of One Mother investigates the intercultural conversations about gender that shaped Anglo-Indian diplomacy. LeMaster's study contributes importantly to historians' understanding of the role of cultural differences in inter group contact and investigates how gender became part of the ideology of European conquest in North America, providing a unique window into the process of colonisation in America.